Metal-Conjugated Affinity Labels: A New Concept to Create Enantioselective Artificial Metalloenzymes

Metal-Conjugated Affinity Labels: A New Concept to Create Enantioselective Artificial Metalloenzymes

Metal-Conjugated Affinity Labels: A New Concept to Create Enantioselective Artificial Metalloenzymes
T. Reiner, D. Jantke, A.N. Marziale, A. Raba, J. Eppinger
ChemistryOpen, Volume 2, Issue 2, Pages 50-54, (2013)
T. Reiner, D. Jantke, A.N. Marziale, A. Raba, J. Eppinger
Affinity labels, Artificial metalloproteins, Asymmetric catalysis, Cysteine proteases, Hydrogenation
2013

How to train a protein: Metal-conjugated affinity labels were used to selectively position catalytically active metal centers in the binding pocket of proteases. The resulting artificial metalloenzymes achieve up to 82% e.r. in the hydrogenation of ketones. The modular setup enables a rapid generation of artificial metalloenzyme libraries, which can be adapted to a broad range of catalytic conditions.

 
 
 
 
10.1002/open.201200044
21911363