Synthetic strategies for efficient conjugation of organometallic complexes with pendant protein reactive markers

Synthetic strategies for efficient conjugation of organometallic complexes with pendant protein reactive markers

Synthetic strategies for efficient conjugation of organometallic complexes with pendant protein reactive markers
D. Jantke, A.N. Marziale, T. Reiner, F. Kraus, E. Herdtweck, A. Raba, J. Eppinger
Journal of Organometallic Chemistry, Volume 744, Pages 82-91, (2013)
D. Jantke, A.N. Marziale, T. Reiner, F. Kraus, E. Herdtweck, A. Raba, J. Eppinger
Bioinorganic chemistry, Metalloenzymes, Peptide coupling, Protease inhibitors
2013


Site-directed conjugation of metal centers to proteins is fundamental for biological and bioinorganic applications of transition metals. However, methods for the site-selective introduction of metal centers remain scarce. Herein, we present broadly applicable synthetic strategies for the conjugation of bioactive molecules with a range of organometallic complexes. Following three different synthetic strategies, we were able to synthesize a small library of metal conjugated protein markers featuring different types of protein reactive sites (epoxides, phenylphosphonates, fluorosulfonates and fluorophosphonate groups) as well as different late transition metals (iron, ruthenium, rhodium, palladium and platinum). The products were isolated in moderate to excellent yields and high purity. Furthermore, X-ray diffraction of the metalated protein markers corroborates structural integrity of the metal complex and the protein reactive site.




10.1016/j.jorganchem.2013.05.027

0022328X